![]() ![]() So now, we're gonna have aįilter with these fragments and the filter is a lot What we'll do is we'll take a filter that's basically the same size as the gel and we're gonna basically just put it right on top of the gel for a little bit and the fragments will basically transfer on to the filter. So, we want to transfer it onto a filter. And what the filter willīasically allow us to do is it allow us to visualize 'cause this gel is very flimsy. So the next step, step number three is basically we're gonna take this gel and we're gonna transfer it to a filter. So now, we've got this gel and we've got the DNA fragments separated by size on this gel. So, we're gonna have theseįragments separated like so. Gonna move down the gel and they're gonna basically be separated based on size and based on charge. So, let's imagine that this is the gel and we add the DNA fragments So, we're gonna take these DNA fragments and we're gonna run them on a gel. But basically, the gel electrophoresis will help us separate these DNA fragments based on size and based on charge. And I made a video on gel electrophoresis if you want to refresh, you can watch that video. So, specifically we're gonna do a gel electrophoresis, "electrophoresis" on these DNA fragments. Now Step 2, what do we do? Well, what we're gonna do is we're gonna take all these tiny little DNA fragments and we're gonna run them on the gel. And that will result in lots of these smaller pieces of DNA. We're gonna expose it to enzymes that will basically cleave the DNA in a whole bunch of different parts. So, we got this big strand and we're gonna cut it up. So, we're gonna take this big old strand. So, "take the DNA and cleave it." So, let me draw that out. So Step 1, what we're gonna do is we're gonna take this DNA and we're gonna cleave this. And we'll break it up intoĪ couple of different steps. Now, in order to figure out whether or not Gene A is inside this cup, basically we have to do this process known as a Southern Blot. If it's inside of this long piece of DNA. I'm interested in Gene A and I want to see if Gene A is inside of this cup. And there's just lotsĪnd lots of those DNA and let's imagine that I'm specifically interested in one gene. So it's got just a wholeīunch of DNA inside. So let's imagine that we have a cup and it's filled with DNA. So, a Southern Blot basically allows you to visualize a specific piece of DNA that you're interested in. Gonna be talking about something known as a Southern Blot. ![]()
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![]() ![]() The developers found creative ways to make seemingly normal plants into weaponized flora, and the results are instantly endearing. ![]() On the plant side of things, you have the Peashooter, Laser Bean, and Sunflower. Zombies doesn’t have any human heroes the way Starcraft has Tychus and Jim Raynor, but it does have a myriad of hilarious and iconic units. Zomboss lives, and he’ll have a surprise for you at the end of each level. There are also multiple challenges like the endless levels, mini-games, and also the daily Pinata Party challenge. With over 300 levels, you’ll become well-acquainted with each and every setting!īut the gameplay doesn’t end at just those levels. Learn more about the next PvZ mobile title from PopCap Games Executive Producer, Bruce Maclean. Learn more about the latest updates of the next PvZ mobile title from PopCap Games Executive Producer, Bruce Maclean. Power Mints are your plants’ best friends on the lawn (besides you). The most enjoyable zombies return to Android. This journey to try to get back to their proper time plays out through 11 crazy worlds that will take you from the aforementioned Ancient Egypt, all the way to the Far Future, with so many stops in between. Read the latest update from Matt Townsend about the next PvZ game. Download the latest version of Plants Vs Zombies 2 for Android. Now, the player must battle zombies from across time until they can find a way to get back to their proper time period. As you can probably imagine, this little scheme takes a turn for the worst, and the gang gets thrown 4,000 years into the past. Upon finishing the taco and realizing how delicious it was, Crazy Dave decides he wants to eat it again!īut, instead of just getting another taco, he calls up Penny, a time-traveling car, to take him back in time to eat that very same taco. ![]() Zombies, everyone’s favorite unhinged character, Crazy Dave, takes a minute to enjoy a delicious taco. Zomboss at the end of the original Plants vs. A Hilarious Setupįollowing the successful defeat of Dr. Zombies and you want some more of that colorful, tactical action, then here is everything you need to know before you dig into Plants Vs. Zombies, one of the craziest and funniest tower-defense of all time. Zombies 2: It's About Time), you will enjoy the sequel of Plants vs. This second entry in one of the most popular video game series of the past decade illustrates the constant lock struggle between the brain-munching undead and flora of the garden by having zombies from different time periods show up to aid the offensive.ĭeveloped by EA, Plants Vs Zombies 2: It’s About Time takes the wildly successful tower defense formula established in the first game and improves upon it in a number of ways. Version 10.3.1 License Freeware Language en Fiches Downloads Video games Strategy Yoann Noail 28/04/23 15:16 In Plants vs. PvZ2: Reflourished is out now Heres how to install the mod and play it for yourself, in case youre new to PvZ2 modsNOTE: The official link has two differ. One such rivalry is the one contained within Plants Vs Zombies 2: It's About Time. The upload that was on here just so happened to have the same errors as my version did from 3 years ago, as well as the same tracklist and the same songs missing in the last "public" version of the soundtrack, so I figured someone found that version and reuploaded it here, and because I felt like it, I submitted the fixed version on here.Some rivalries are as old as time, stretching across generations with no conceivable ending. Figured it'd be easier than to replace the new tracks, cause frankly I don't even remember what tracks I fixed in the newest version anymore lol. I uploaded that "fixed" version here in its entirety. I did upload a newer version but it was deleted by the mods on the sub. Zombies 2 old version APK for Android Download About Plants vs. This is the only available version of my "sequencing" that exists on the internet rn. Wait a second, i had uploaded this album back in may of last year, why does it say somebody else is the creator of the upload?įar as I could tell, you used this link as a base, correct? Angry Birds Don’t miss out on another exciting zombie game with Pants vs Zombies 2 for PC. ![]() ![]() ![]() ![]() In addition to ad hoc planning based on customer requests, SAP service solutions also support the planned execution of service tasks. Requests in the system may contain information about what should be performed on which object, what resources are needed to process the request (materials, tools, service engineers or third parties), as well as the cost and revenue accounting method (internal costs or customer billing), etc. Planning and processing the service tasks, both warranty and non-warranty, by using different types of service requests is another service functionality. Additionally, the system enables the codification of the reported problems by using definable code catalogues that describe the problems, reasons, effects, actions to be taken, etc. Within the two solutions, you can use the so-called solution database, enabling you to quickly identify the reported problem and propose a solution based on the reported symptoms. This allows for rapid identification of customers calling and the use of the advanced features of telephone exchanges (recording calls, calling back to the customer, etc.) Both systems allow the registration of service requests through the use of different types of documents (types of service requests) or through the call centre functionality and its integration with telephone exchanges. Request management is another process which is essential for customer services. The main system objects used for this purpose include: installations, functional locations, equipment, components, materials with serial numbers. Service tools also allow you to map and manage customer technical objects used to carry out the service activities. equipment, spare parts, services) as well as warranty data (conditions, dates, coverage). The most important ones include mapping and management of master data, that is, information on customers, employees (responsible for customer relationships, service engineers, etc.) and materials (e.g. Basic functions – similaritiesīoth SAP CS and the service module in SAP CRM support a wide range of business processes implemented by the departments responsible for providing the services. By using interfaces, it is possible, for example, to integrate them with GIS (geographic information systems that allow of choosing an optimal route for service technicians), to transmit service requests to the technicians’ devices (laptops, palmtops, PDAs) or to access the module through web browsers and portals. Each of these tools gives access to the functionality supporting the business processes at all stages for companies providing customer services – from reporting the problem, through planning the works and their execution, to the invoice settlement and issuance.īoth solutions provide flexibility, allowing full integration with other SAP systems (ERP, BI), and also with external applications. These are SAP Customer Service (CS module of the ERP system) and SAP CRM Service. SAP offers two solutions that can be used to meet the needs in the service area. Therefore, a suitable data source is necessary for making service-related decisions and for their efficient processing. The reaction of the customer service, however, depends on the information flow rate and information accuracy. One of the most important characteristics required from the service department is the speed of reaction allowing you to meet customers’ requirements, and thus gain their trust. ![]() ![]() ![]() Very convenient for dining and the occasional run up to the general store. Loved the private transpiration within the Kapalua resort. A premier Maui resort, Montage Kapalua Bay invites guests to experience oceanfront accommodations imbued with island spirit. Very helpful, always addressing us by name. In room dining food and service excellent. Also, I found it odd the entire top level of the pool complex isn't adults only since there is a hot tub and a little lagoon area to the right of the Serenity pool which was full of families and loud kids which made the Serenity pool not so serene. After enjoying a candy-colored Maui sunset and postcard-perfect views of the Pacific from their ocean suite, couples can head to the hotels historic Cliff. Loved the adults only pool but no staff seemed to do anything to limit kids access so there were occasionally kids screaming through the pool. Additional amenities at this hotel include. Towels poolside were the only thing I found lacking, stained and small. Youre sure to appreciate the recreational amenities, including an outdoor pool, a spa tub, and a sauna. Loungers around the pool were outstanding. ![]() Formed in the shape of a half-moon, it offers a superb white sandy beach and a. Very private with lots of hidden lagoons. Scenic Kapalua Bay lies on the far northwestern shore of the Island of Maui. ![]() Recently renovated, guests at this 24-acre beach resort in Maui will. With spacious suites that offer full kitchens. Montage Kapalua Bay Residences: amenities include a gourmet Hawaiian restaurant. The pool is the most amazing pool we've ever seen. Montage Kapalua Bay is a luxury resort that provides a tranquil escape in the picturesque Kapalua Bay. Coffee was very good provided in the room with a very nice carafe style coffee maker. We could have cooked complex meals and would have had everything we needed. Beautiful decor, comfy furniture, and especially the amazing balcony with daybed, loungers and large dining table. The 2 bedroom ocean-view villa was exceptional. Our new favorite Hawaiian resort by far and maybe the best tropical location resort we've visited. ![]() ![]() ![]() You may be wondering: Is well-being the same as wellness? The two terms are often used interchangeably, especially in holistic corporate wellness programs.īut if you want to dig deeper, it pays to know the subtle differences between the two: WELL-BEING RELATED: 14 Reasons Why Many Corporate Wellness Programs Fail The Difference Between Well-Being and Wellness And sometimes the process is reversed-stress or dissatisfaction at work can often negatively impact otherwise physically and mentally healthy employees. Employees who are not of sound mind or body will naturally experience compromised well-being. ![]() Physical health and mental health are integral components of workplace well-being. There are many examples of bad working conditions-a common one is improper space utilization in the office, which leads to discomfort, claustrophobia, and lack of privacy.Įmployees may be forced to deal with non-ergonomic office equipment, excessively noisy workstations, inadequate lighting, too cold or too hot offices, smelly surroundings, or unclean facilities. One common demotivator for employees is poor workplace conditions. workstations that limit or discourage face-to-face interactions,īad working conditions.This refers to a lack of friendships or social ties, also known as workplace loneliness. Financial stress can also lead to depression, insomnia, weight loss or weight gain, feelings of shame, social isolation, and excessive frugality. This is a specific type of stress characterized by feelings of worry or anxiety over money, expenses, or mounting debts. Burnout is no surprise when an employee is doing much more than what one person can realistically handle.įinancial stress. Work stress can also be caused by improperly set deadlines due to poor planning or delegation by management. Workloads can become overwhelming if the company is understaffed. Let us look at some of them:īurnout due to excessive workload. Many factors may threaten an employee’s well-being. Relationships with colleagues or with the team.According to the organization, workplace well-being involves: The Chartered Institute of Personnel and Development (CIPD) offers a more detailed outline of what constitutes workplace well-being. RELATED: 17 Employee Wellness Program Ideas to Increase Engagement Importance of Workplace Well-Being Activities They must also feel safe, satisfied, and engaged as they work. The ILO further states that for an employee to achieve optimal workplace well-being, they must be physically and mentally healthy. The organization’s hierarchy and management. ![]()
![]() ![]() That also means your character will need to practice safe sex for the duration of the challenge if you want to avoid having kids. With that in mind, there’s no need to enter a relationship or have children if you want to complete this challenge - if anything, having a family could cut into your bank balance and net worth. However, there is one option that you can practically spam by repeating it several times per year, starting from the time you turn 18, and that’s the Hook Up option in the Love sub-menu under Activities. This would include all types of lovers regardless of gender identity or sexual orientation, but the fact that you’re going to need to have at least a hundred of them may make this a bit time-consuming. One of the four requirements of the Rock Star Challenge is to sleep with a minimum of 100 lovers. The 100 Lovers Requirement – Use The Hook Up Option Freely, But Play It Safe As usual, you may have to quit the app and restart it repeatedly before the opening becomes available in the Jobs menu. But for the purposes of completing this challenge as quickly as possible, we recommend getting that Background Vocalist job right after graduating from (or dropping out of) high school. If you’d rather have a fallback in case rock stardom doesn’t work out, it’s also your call if you wish to stay well-behaved and attend college before starting your career. You can even rack up a criminal record as a juvenile or drop out once you reach the legal age to do so and it won’t matter - the Background Vocalist job does not require any diploma nor does it disqualify those with a criminal record. The stereotypical rock star, after all, isn’t exactly the scholarly type, so you can act up in class, fight back against bullies, mess with classmates, or play hooky as you please. When living out your formative years, there aren’t any restrictions that would require you to stay out of trouble and/or get good grades in school. This challenge is open to male or female Bitizens, so you don’t need to pay attention to gender either - again, it’s all about Looks when it comes to character creation. Based on our experience, a Smarts stat of 40 or above is necessary in order to get an entry-level job in the Pop Star career track, but you need not worry too much about this stat - if you don’t have God Mode so you can edit your stats before your character is born, we would suggest re-rolling until you get a character with 80 Looks or better. ![]() When creating a new character for the Rock Star Challenge, there is one stat that you should be prioritizing above everything else, and that’s Looks. Creating Your Character – Smarts Are Optional, Looks Are Everything So without further ado, here’s our BitLife mini guide for quickly completing the Rock Star Challenge. The challenge we are referring to is the Rock Star Challenge, and this is your chance to unleash your inner Mick Jagger…or Adam Levine, if you prefer newer rock stars who’ve got the “moves like Jagger.”īut it’s more than just becoming famous in here, as you’ll also need to live up to another third of the “sex, drugs, and rock ’n’ roll” maxim, and it’s not the one that has something to do with getting wasted. ![]() Personally, the latest BitLife challenge was quite an easy one for us, and it could be for you too, if you’re the type of player who wants to turn their Bitizens into celebrities. This feature is available on both iOS and Android versions of the game, and the latest such challenge to go live should be a treat for anyone who may have, at specific points in their lives, wanted to be a rich and famous musician. As most BitLife players should know by now, it was several months ago when its maker, Candywriter, introduced the concept of challenges, which are limited-time events that require players to create a character whose life moments tick certain boxes and fulfill certain requirements based on a given theme. ![]() ![]() ![]() They've kept SRH in the game despite the wickets.ĥ0 for Tripathi- his third this season. Pooran departs for 38 off 22.ĥ0-run stand:Nicholas Pooran and Tripathi have revived SRH after Garg fell for 42. Great catch in deep! OUT!Markande picks up a great diving catch off Pooran's flick and the 76-run stand is broken. Tripathi skies one to Tilak Varma for 76 off 44.SRH needed him if they wanted to get to 200, but with two new batsmen in the middle, we'll see how many they can get. Spectacular for the 18th as MI pull things back. Two wickets in the over and Markram departs!Just 2 runs and 2 wickets in the over. Can MI chase this down? We'll find out in a bit. SRH managed to score 45 runs in the last 5 and would feel they could've got a few more had Tripathi and Pooran stuck on for a bit longer. They looked set to get a big total, but MI did well to pull things back, even if it's a tad bit. Garg fell for 42, but Tripathi and Pooran joined hands for a dynamic stand to take SRH over 150. SRH lost Abhishek Sharma early, but Tripathi and Priyam Garg, who was brought in to open with Abhishek, did well to revive the innings with a 78-run stand. Given that they were well on their way to get there in the 17th over that the set batsmen Tripathi and Pooran fell in successive overs, pegged them back as they finished with 193 on the board. MI have done well to restrict SRH to a total below 200. They're rattling along at close to nine an over inside the powerplay. Quick start for MI.Both Ishan Kishan and Rohit Sharma, who's hit two sixes off Bhuvneshwar and one scoop off Natarajan, have found the boundaries regularly. The openers have added 50 runs for the opening wicket He ends up with a mistime to deep midwicket where substitute fielder Suchith takes the catch. Washington strikes!Rohit falls for 48 off 36 as SRH have broken the 95-run opening stand finally! Rohit came down the track as Washington pulls back the length. Stat: Still no 100+ opening partnership involving Rohit Sharma in IPL. OUT! Umran Malik strikes and gets Ishan.Both openers got starts but couldn't make them count, falling in successive overs. Umran has another!This time it's Tilak Varma, who is late on the pull and ends up with a slices to extra cover where Williamson grabs it. MI need 44 in 18 balls.Ģ in the over for Umran and SRH!Daniel Sams falls after a brilliant catch from Garg. Run-out!Tristan Stubbs has been caught short of his crease as MI lose their fifth. However, he's run out on the final ball for 46 off 18 balls. OH NO!Tim David smashed T Natarajan for 26 runs in the over, including 4 sixes, to take MI within touching distance of the finish line. Yadav falls as SRH keep chipping in with wickets. The 19th over is a maiden wicket by Bhuvneshwar. Do join us tomorrow when LSG take on KKR. They eventually fell just short and are rooted to the bottom of the table. ![]() David kept MI in the chase, but once he fell, it was curtains. SRH kept chipping in with wickets at crucial times, with Umran Malik picking two in an over. The pair added 95 runs for the opening wicket before falling in successive overs. MI would've expected themselves to chase this down, given the start they got from Rohit and Ishan. They're now tied with KKR and PBKS on 12 points, but are still on 8th, behind on NRR. SRH held their nerve well to finish on the winning side and pick up two crucial points. Bhuvneshwar Kumar's 19th over was spectacular - bowling a wicket maiden with yorkers galore as SRH tightened the screws. However, he was run out on the final ball of that over to give SRH the advantage. He bridged the gap too with a 26-run over off Natarajan in the 18th. Until Tim David was in the middle, MI were in with a chance. SRH KEEP CAMPAIGN ALIVE! They've ended their 5-match losing streak.Mumbai Indians have fallen short by 3 runs, just 3 runs. ![]() ![]() This again was more strongly true of the many nights of local festivity, when the little gardens were often illuminated, and the big Chinese lanterns glowed in the dwarfish trees like some fierce and monstrous fruit. More especially this attractive unreality fell upon it about nightfall, when the extravagant roofs were dark against the afterglow and the whole insane village seemed as separate as a drifting cloud. A man who stepped into its social atmosphere felt as if he had stepped into a written comedy. He had not discovered anything new in biology but what biological creature could he have discovered more singular than himself? Thus, and thus only, the whole place had properly to be regarded it had to be considered not so much as a workshop for artists, but as a frail but finished work of art. That scientific gentleman with the bald, egg-like head and the bare, bird-like neck had no real right to the airs of science that he assumed. That old gentleman with the wild, white beard and the wild, white hat-that venerable humbug was not really a philosopher but at least he was the cause of philosophy in others. That young man with the long, auburn hair and the impudent face-that young man was not really a poet but surely he was a poem. Even if the people were not “artists,” the whole was nevertheless artistic. The place was not only pleasant, but perfect, if once he could regard it not as a deception but rather as a dream. Nor when he met the people was he disappointed in this respect. The stranger who looked for the first time at the quaint red houses could only think how very oddly shaped the people must be who could fit in to them. But although its pretensions to be an intellectual centre were a little vague, its pretensions to be a pleasant place were quite indisputable. It was described with some justice as an artistic colony, though it never in any definable way produced any art. ![]() It had been the outburst of a speculative builder, faintly tinged with art, who called its architecture sometimes Elizabethan and sometimes Queen Anne, apparently under the impression that the two sovereigns were identical. It was built of a bright brick throughout its sky-line was fantastic, and even its ground plan was wild. The suburb of Saffron Park lay on the sunset side of London, as red and ragged as a cloud of sunset. Sample from The Man Who Was Thursday, by G. Or, if you are not ready to install, try Converter. Install the bookmarklet in your browser and build an e-book from the sample text below, just clicking on the installed bookmarklet. with a single click!Īnd, because it is software in the cloud, you benefit from the improvements of future releases without having to re-install anything. Save now and immersively read later (even offline) those long and deep articles you didn’t have time to read while browsing.īuild a personal library of your favorite blog posts, news articles, etc.Ĭompatible with the iPad, the iPhone, the iPod Touch, the Sony Reader, the Nook, the iLiad, the BeBook, the Cool-er, the CyBook, the Alex eReader, the Kobo eReader, the Elonex eBook, the eSlick, the eClicto, the Hanlin eReader, the QUE ProReader, the Papyre, the Leqtor.įinally, infinite content for your e-reader. Featuresĭownload webpages to any epub-compatible device: e-readers, tablets, smartphones, netbooks, desktop computers. And, if you have a blog, we provide a WordPress plugin that automatically embeds our widget.įor more customizable results, developers can use our API (read the documentation). The widget helps your users getting the content of your website in e-book form (see the tips for webmasters). Creator lets you make an e-book from a text of your own. And, if you are a Chrome, Firefox, Edge or Safari for Mac user, you can install the dotepub extension in your browser.įor content producers (editors, authors), we offer Creator and a widget. dotepub requires a Javascript-enabled browser!ĭotepub is software in the cloud that allows you to convert any webpage into an e-book.įor content consumers (readers), we have developed a bookmarklet for modern browsers (desktop or mobile). ![]() Your contribution allows us to continue its development. If, after trying dotepub, you still like it, please consider making a donation. Visit this page with your device to see the installation and use instructions. Watch the videos! iPad, iPhone & iPod Touch To get better results, use the print version of the webpage (visit the FAQ page for more answers). Just press the dotepub bookmark in your browser’s toolbar. Visit an article webpage you want to save or transfer to your e-reader as an e-book. Copy and paste this code into a new bookmark ↓ javascript:(function())() Use The bookmarklet can be created manually: you just need to copy the code below into the address field (URL) of a new bookmark (also known as favourite). ![]() ![]() ![]() Valid values are minutes, hours, days, weeks, months and copies. ![]() Valid values are 1 and 0 with 1 = TRUE and 0 = additional verify options as documented in BOL for the VERIFY WITH unit of measure for the parameter. Sub folders will be created for each whether to verify the backup file. REORG - Reorganize all additional backup options as documented in BOL for the BACKUP WITH base folder to write the backups to.Valid values are a single database name, ALL_USER which will process all user databases and ALL_SYSTEM which will process all system type of maintenance operation to be performed. The following table describes the available parameters in this stored target database for the maintenance operation. This will install expressmaint stored procedure provides the following features: You can install the stored procedure by executing the sql_express_maintanance.sql file on your SQL Server (on the Master DB, system databases/master) with a user that has sysadmin rights. Installing the stored procedure for maintenance ![]() In this document we explain how to use these two parts to create you database backup solution. We recommend Windows Task Scheduler to run the maintenance tasks Verba provides a stored procedure, called expressmaint (comes installed with the product) There are two parts of the backup solutions for Express Edition: SQL Server 2005/2008 Express Edition does not contain maintenance plans and SQL Server Agent service. Database backup with SQL Server Express Edition ![]() |
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